mouse anti il1β  (Santa Cruz Biotechnology)

Journal: Frontiers in Immunology

Article Title: TGFβ1 attenuates microglial IL1β release through inhibition of NLRP3 inflammasome priming

doi: 10.3389/fimmu.2025.1623643

Figure Lengend Snippet: TGFβ1 significantly reduces LPS-induced increases in NLRP3, CASP1, and IL1β protein levels in BV2 cells. After treatment with TGFβ1 (5 ng/ml), LPS (1 µg/ml) or the combination of both factors for 6 h and 12 h, protein levels were analyzed using western blotting. Representative western blot images for both experimental time points are depicted for NLRP3 (A) , CASP1 (C) , and IL1 β (E) . Quantifications after normalization using β-Actin are presented for NLRP3 (B) , CASP1 (D) , and IL1β (F) . Data are given as means ± SEM from at least three independent experiments. P-values derived from one-way ANOVA followed by Tukey´s multiple comparison tests are * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001.

Article Snippet: They were later incubated with primary antibodies against Il1β (AF-401-NA, R&D Systems, 1:500), Nlrp3 (AG-20B-0014, Adipogen, 1:500), Caspase-1 (AG-20B-0042, Adipogen, 1:500) and β-Actin (#4967, Cell Signaling,1:2000) overnight at 4 °C.

Techniques: Western Blot, Derivative Assay, Comparison

Journal: Frontiers in Immunology

Article Title: TGFβ1 attenuates microglial IL1β release through inhibition of NLRP3 inflammasome priming

doi: 10.3389/fimmu.2025.1623643

Figure Lengend Snippet: TGFβ1 inhibits LPS-induced expression of Nlrp3 , Casp1 , Il18 , and Il1b in primary microglia. Microglia were treated with TGFβ1 (5 ng/ml), LPS (1 µg/ml) or the combination of both factors for 6 h and RNA and total proteins were isolated. TGFβ1 significantly inhibits LPS-induced upregulation of Nlrp3 (B) , Casp1 (B) , Il18 (C) , and Il1b (D) in primary microglia. TGFβ1 abrogates LPS-induced increases in NLRP3, CASP1, and IL1β protein levels in microglia. Representative western blot images are depicted (E) . Quantifications after normalization using β-Actin are presented for NLRP3 (F) , CASP1 (G) , and IL1β (H) . Data are given as means ± SEM from at least three independent experiments. P-values derived from one-way ANOVA followed by Tukey´s multiple comparison tests are * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001.

Article Snippet: They were later incubated with primary antibodies against Il1β (AF-401-NA, R&D Systems, 1:500), Nlrp3 (AG-20B-0014, Adipogen, 1:500), Caspase-1 (AG-20B-0042, Adipogen, 1:500) and β-Actin (#4967, Cell Signaling,1:2000) overnight at 4 °C.

Techniques: Expressing, Isolation, Western Blot, Derivative Assay, Comparison

Journal: Frontiers in Immunology

Article Title: TGFβ1 attenuates microglial IL1β release through inhibition of NLRP3 inflammasome priming

doi: 10.3389/fimmu.2025.1623643

Figure Lengend Snippet: TGFβ1 reduces release of inflammasome cleaved IL1β from primary microglia. Primary microglia were treated with TGFβ1 (5 ng/ml), LPS (1 µg/ml) or the combination of both factors for 6 h and inflammasome assembly was triggered using Nigericin (1.34mM/ul) treatment for additional 2 h. Proteins were isolated from whole cell lysates (WCL) and supernatants and pro- IL1β and cleaved IL1β was visualized. Representative western blot images are depicted (A) . Quantifications of pro-IL1β after normalization using β-Actin is presented (B) . Total levels of cleaved and bioactive IL1β in supernatants were quantified using ELISA. Quantifications of released IL1β are given (C) . Data are given as means ± SEM from at least three independent experiments. P-values derived from one-way ANOVA followed by Tukey´s multiple comparison tests are ** p < 0.01, and **** p < 0.0001.

Article Snippet: They were later incubated with primary antibodies against Il1β (AF-401-NA, R&D Systems, 1:500), Nlrp3 (AG-20B-0014, Adipogen, 1:500), Caspase-1 (AG-20B-0042, Adipogen, 1:500) and β-Actin (#4967, Cell Signaling,1:2000) overnight at 4 °C.

Techniques: Isolation, Western Blot, Enzyme-linked Immunosorbent Assay, Derivative Assay, Comparison